Cocoa pods, Venezuela. Photo: C.Lanaud ©CIRAD
Arabica coffee, Ethiopia. Photo: ©Jean-Pierre Labouisse
Yams in Benin. Photo: J-L Pham ©IRD
Rice harvest, Guinea. Photo: J-L Pham ©IRD
Maize corn. Photo: ©Brigitte Gouesnard

Pangenomic study of diversity through the reduction of genome complexity

The phrase « genotyping by sequencing » or GBS, which recently emerged in the scientific literature, is a generic term covering several genotyping procedures which make direct use of DNA sequence information for genetic diversity analyses. Obtaining the full genomic sequence of all the individuals in a population is still a difficult task in many species. Methodologies have thus been developed to sequence an identical, restricted section of the genome in each of the individuals.

ARCAD aims to implement various strategies for reducing genomic complexity in our species. The strategies are to be selected depending on the pending genetic questionand the complexity of the genome. Several procedures, all adapted to NGS techniques, can be used to reduce genomic complexity in order to study only a targeted part of the genome. In the Pangenomic study of diversity project we aim to master, adapt and apply a method using restriction enzymes to select a section of the genome.

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