Cocoa pods, Venezuela. Photo: C.Lanaud ©CIRAD
Arabica coffee, Ethiopia. Photo: ©Jean-Pierre Labouisse
Yams in Benin. Photo: J-L Pham ©IRD
Rice harvest, Guinea. Photo: J-L Pham ©IRD
Maize corn. Photo: ©Brigitte Gouesnard

Cryopreservation

 Papers

  • Engelmann-Sylvestre I, Engelmann F. Cryopreservation of in vitro-grown shoot tips of Clinopodium odorum using aluminium cryo-plates. Scientia Horticuturae, submitted.
  • Marković Z,  Preiner D, Bošnjak AM, Safner T, Stupić D, Andabaka Z, Maletić E, Chatelet P, Engelmann F, Kontić JK. 2014. In vitro introduction of healthy and virus-infected genotypes of native Croatian grapevine cultivars. Central European Journal of Biology 9:1087-1098 (DOI) 10.2478/s11535-014-0337-7.
  • Marković Z, Chatelet P, Preiner D, Sylvestre I, Engelmann F, Karoglan Kontić J. 2014. Effect of shooting media and source of material of grapevine shoot tip (Vitis vinifera) recovery after cryopreservation. CryoLetters 35: 40-47.
  •  Barraco G, Sylvestre I, Collin M, Escoute J, Lartaud M, Verdeil JL, Engelmann F. 2014. Histological study of yam shoot tips during their cryopreservation using the encapsulation-dehydration technique. Protoplasma 251: 177-189. doi.org/10.1007/s00709-013-0536-5.
  • Marković Z, Chatelet P, Sylvestre I, Karoglan Kontić J, Engelmann F. 2014. Cryopreservation of grapevine (Vitis vinifera L.) in vitro shoot tips using encapsulation-dehydration and droplet-vitrification. Central European Journal of Biology 8: 993-1000.
  • Salma M, Sylvestre I, Collin M, Escoute J, Lartaud M, Yi JY, Kim HH, Verdeil JL, Engelmann F. 2014. Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots. Protoplasma 251:649-659. DOI 10.1007/s00709-013-0565-0.
  • Polzin F, Sylvestre I, Déchamp E, Ilbert P, Etienne H, Engelmann F. 2014. Multiplication of African yam (Dioscorea cayenensis-rotundata) using a temporary immersion system. In Vitro Cellular and Developmental Biology – Plant 50: 210-216. DOI 10.1007/s11627-013-9552-6.
  • Engelmann-Sylvestre I, Engelmann F. 2014. Effect of various growth regulators on growth of yam (Dioscorea trifida) in vitro shoot tips. African Journal of Biotechnology 13: 1645-1649.
  • Engelmann-Sylvestre I, Engelmann F. 2013. Effect of brassinosteroids on growth of in vitro shoot tips of several yam (Dioscorea spp.) species. American Journal of Plant Sciences 4: 2271-2274. doi.org/10.4236/ajps.2013.411280.
  • Marković Z, Chatelet P, Peyrière A, Preiner D, Engelmann-Sylvestre I, Karoglan Kontić J, Engelmann F. 2013. Effect of proline pretreatment on grapevine shoot-tip response to a droplet-vitrification protocol. American Journal of Plant Sciences 4:2414-2417.
  • Barraco G, Chatelet P, Balsemin E, Sylvestre I, Engelmann F. 2012. Cryopreservation of Prunus cerasus using vitrification method and replacement of cold hardening with preculture on medium enriched with sucrose and/or glycerol. Scientia Horticulturae 148:104-108. doi:10.1016/j.scienta.2012 .09.034
  • Barraco G, Sylvestre I, Iapichino G, Engelmann F. 2013. Investigating the cryopreservation of nodal explants of Lithodora rosmarinifolia (Ten.) Johnst., a rare, endemic Mediterranean species. Plant Biotechnology Report 7:141-146. doi: 10.1007/s11816-012-0241-4
  • Yi JY, Sylvestre I, Collin M, Salma M, Lee SY, Kim HH, Park HJ, Engelmann F. 2012. Improved cryopreservation using droplet-vitrification method and histological changes associated with cryopreservation of madder (Rubia akane Nakai). Korean Journal of Horticultural Science and Technology 30:79-84. doi: 10.7235/hort.2012.11087
  • Barraco G, Chatelet P, Balsemin E, Sylvestre I, Engelmann F. 2012. Cryopreservation of Prunus cerasus using vitrification method and replacement of cold hardening with preculture on medium enriched with sucrose and/or glycerol. Scientia Horticulturae 148 :104-108. doi:10.1016/j.scienta.2012.09.034
  • Vujovic T, Sylvestre I, Ruzic D, Engelmann F. 2011. Droplet-vitrification of apical shoot tips of Rubus fruticosus  L. and Prunus cerasifera  Ehrh. Scientia Horticulturae 130:222-228. doi:10.1016/j.scienta.2011.06.049
  • Barraco G, Sylvestre I, Iapichino G, Engelmann F. 2011. Cryopreservation of Limonium serotinum apical shoots from in vitro plantlets using droplet-vitrification. Scientia Horticulturae 130:309-313. doi:10.1016/j.scienta.2011.07.001
  • Barraco G, Sylvestre I, Engelmann F. 2011. Cryopreservation of sugarcane (Saccharum spp.) shoot tips using encapsulation and droplet-vitrificationScientia Horticulturae 130 : 320-324. doi:10.1016/j.scienta.2011.07.003

Conferences

  • Barraco G, Sylvestre I, Collin M, Escoute J, Lartaud M, Verdeil JL, Engelmann F. Histological study of yam shoot tips during their cryopreservation using the encapsulation-dehydration technique. 2nd Intl. Symp. on Plant Cryopreservation. Fort Collins, USA, 11-14 August 2013, submitted.
  • Marković Z, Chatelet P, Sylvestre I, Karoglan Kontić J, Engelmann F. Cryopreservation of grapevine (Vitis vinifera L.) in vitro shoot tips using encapsulation-dehydration and droplet-vitrification. 2nd Intl. Symp. on Plant Cryopreservation. Fort Collins, USA, 11-14 August 2013, submitted.
  • Salma M, Sylvestre I, Collin M, Escoute J, Lartaud M, Yi JY, Kim HH, Verdeil J L, Engelmann F. Effect of the successive steps of a cryopreservation protocol on the structural integrity of Rubia akane Nakai hairy roots. 2nd Intl. Symp. on Plant Cryopreservation. Fort Collins, USA, 11-14 August 2013, submitted.
  • Barraco G, Sylvestre I, Engelmann F. 2012. Cryopreservation of sugarcane (Saccharum sp.) shoot tips using encapsulation-dehydration and droplet-vitrification. In : A. Grapin, E.R.J. Keller, P.T. Lynch, B. Panis, A. Revilla Bahillo & F. Engelmann (eds.), Cryopreservation of crop species in Europe Proceedings of the final meeting, Agrocampus Ouest INPH, Angers, France, 8-11 Feb. 2011, COST Office, Brussels, pp. 119-122.
  • Marković Z, Chatelet P, Sylvestre I, Karoglan Kontić J, Engelmann F. 2012. Duration of culture of grapevine (Vitis vinifera) microcuttings on medium with zeatin riboside affects shoot tip recovery after cryopreservation. In : A. Grapin, E.R.J. Keller, P.T. Lynch, B. Panis, A. Revilla Bahillo & F. Engelmann (eds.), Cryopreservation of crop species in Europe Proceedings of the final meeting, Agrocampus Ouest INPH, Angers, France, 8-11 Feb. 2011, COST Office, Brussels, pp. 145-147.
  • Vujović T, Sylvestre I, Ružić DJ, Engelmann F. 2012. Cryopreservation of cherry plum and blackberry shoot tips by droplet-vitrification. In : A. Grapin, E.R.J. Keller, P.T. Lynch, B. Panis, A. Revilla Bahillo & F. Engelmann (eds.), Cryopreservation of crop species in Europe Proceedings of the final meeting, Agrocampus Ouest INPH, Angers, France, 8-11 Feb. 2011, COST Office, Brussels, pp. 163-166.

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