Cryopreservation is a storage technique of biological material at ultra-low temperature, usually that of liquid nitrogen, -196°C. That platform will ensure safe, long-term and cost-efficient storage of genetic resources of species with non-orthodox seeds, vegetatively propagated species, rare and endangered species, and biotechnology products. It is equipped by cryotanks, LN transport tanks, programmable freezer, dewar vessels, thermostated water-bath, binocular microscope, magnetic stirrer, incubators, labelling machine. In France, cryopreservation is not very used for crop genetic resources, while it is used in animals or microorganisms. By disseminating the use of the technique in CRBs network, this platform will become the first National Plant Cryobank.